Lasers
The TCS SP8 provides the following lasers with the specified laser lines for fluorophore excitation:
Blue diode |
405 |
||
| Arg | 458 |
488 |
514 |
| Diode | 561 |
||
| HeNe | 633 |
Objectives
The LSM 510 META is equipped with the following lenses:
| lens | magnif. | corr | NA | WD |
|---|---|---|---|---|
| HC PL APO 10x CS2 | 10x |
A |
0.4 |
2.2 |
| HC PL APO 20x CS2 | 20x |
A |
0.75 |
0.62 |
| HC PL APO 40x CS2 | 40x |
O |
1.3 |
0.24 |
| HC PL APO 63x CS2 | 63x |
O |
1.4 |
0.14 |
| HC PL APO 63x | 63x |
W |
1.2 |
W = water, A = air, O = oil, WD = working distance in mm
Filter sets
Standard filter cubes for epifluorescence detection of DAPI (exc 360/40, em 425 LP), FITC (exc 470/40, em 515 LP) and Rhodamine (exc 540/45, em 590 LP) are available on the TCS SP8.